In this talk, I will give a brief introduction of a newly emerging single-molecule technique, nanopore biosensing. First, I would like to start with the 20 years history of nanopore research including biological nanopores as well as solid state nanopores. Then, based on the efforts have been done in the lab, I will discuss the merits of this high throughput methodology compared to other single molecule techniques. Last, I will focus on my recent efforts on ultrathin nanopore membranes based on 2D materials, especially molybdenum disulfide (MoS2) to elaborate promising potentials for various applications (e.q., DNA nanopore sequencing).