Biophysics

Estimation of relative loss of telomere length between sister chromatids

March 5, 2012.

Lecturer

Prof. MILJENKO HUZAK, Ph.D.
Department of Mathematics, Faculty of Science, University of Zagreb

Date and time

Friday, March 16, 2012.

Location

Seminar F-201, Faculty of Science - Department of Physics, Bijenička cesta 32, Zagreb

Abstract

Telomeres are the physical ends of chromosomes that shorten with each cell division. Since each telomere has a limited shortening potential, understanding the mechanisms of their shortening also enables understanding cellular aging. Among other things, for this purpose, it is important to set up and evaluate an appropriate regression model for the difference in telomere lengths between the longer and shorter telomeres of sister chromatids, relative to the longer telomere. The proposed linear regression model was adapted and tested on three sets of data obtained from human fibroblast cultures aged, respectively, 32, 42, and 52 PD. The following standard statistical methods were used: weighted least squares method for point estimation of model parameters, nonparametric bootstrap method for interval estimation of parameters and sampling distributions of some test statistics, and leave-one-out cross validation method for verification of the proposed model.
The work was carried out in collaboration with: Nikolina Škrobot Vidaček, Andrea Ćukušić, Milena Ivanković, Hrvoje Fulgosi, and Ivica Rubelj (Ruđer Bošković Institute)

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