Biophysics

Phospholipid-Peptide Interactions using Impendance Spectroscopy

January 3, 2003.

Lecturer

ANDREW NELSON
University of Leeds, Leeds, Great Britain

Date and time

Thursday, January 9, 2003 at 11 a.m.

Location

Lecture Hall, III Wing, Ruđer Bošković Institute, Bijenička cesta 54, Zagreb

Abstract

Joint colloquium of the Institute for Marine and Environmental Research and the Croatian Biophysical Society

Summary: This talk describes the use of electrochemical impedance spectroscopy to understand the behavior of phospholipid layers on mercury electrodes as a function of potential and when interacting with biologically-active peptides in solution.

Experiments were carried out using frequencies between 0.1 and 65000 Hertz on coated and uncoated electrodes. The results clarify the properties of the phospholipid layers at different values of the applied field and they help to characterize three consecutive phase transitions. Interactions with gramicidin peptides are quantitative and selective. A general one and two capacitor model has been developed and applied which takes account of frequency dispersion in the capacitor and interfacial problems between the capacitor and the solution resistance.

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