Links – Hrvatsko biofizičko društvo

Croatian biophysical society is an active member of international organizations that have the purpose to promote biophysics.

Distance measurements in the F 0 F 1 -ATP synthase from E. coli using smFRET and PELDOR spectroscopy 08/11/2019
Abstract Fluorescence resonance energy transfer in single enzyme molecules (smFRET, single-molecule measurement) allows the measurement of multicomponent distance distributions in complex biomolecules similar to pulsed electron–electron double resonance (PELDOR, ensemble measurement). Both methods use reporter groups: FRET exploits the distance dependence of the electric interaction between electronic transition dipole moments of the attached fluorophores, whereas […]
Thermodynamics of DNA: heat capacity changes on duplex unfolding 05/11/2019
Abstract The heat capacity change, ΔCp, accompanying the folding/unfolding of macromolecules reflects their changing state of hydration. Thermal denaturation of the DNA duplex is characterized by an increase in ΔCp but of much lower magnitude than observed for proteins. To understand this difference, the changes in solvent accessible surface area (ΔASA) have been determined for unfolding the […]
Experimental determination of second virial coefficients by small-angle X-ray scattering: a problem revisited 31/10/2019
Abstract This investigation examines the validity of employing single-solute theory to interpret SAXS measurements on buffered protein solutions—the current practice despite the necessity to regard the buffer components as additional non-scattering solutes rather than as part of the solvent. The present study of bovine serum albumin in phosphate-buffered saline supplemented with 20–100 g/L sucrose as small […]